In the BioDeNO(x) process for the nitrogen oxides (NOx) removal, sulfide is supposed to exist in the bioreactor mainly because of the bioreduction of sulfite and sulfate. Thus, the potential roles of sulfide should be taken into account in the BioDeNO(x) process. The objective of this work was to investigate abiotic Fe(II)EDTA-NO reduction by sulfide with respect to stoichiometry and kinetics. Batch experiments showed that elemental sulfur and ammonium were the two major products. However, the molar ratio of reduced Fe(II)EDTA-NO to the oxidized sulfide was 1:3.4-1:3.6, a bit higher than the expected value, suggesting the formation of the polysulfide based on the comprehensive analysis. Results also showed that Fe(II)EDTA-NO reduction by sulfide was a first-order reaction with respect to Fe(II)EDTA-NO. The reduction rates of Fe(II)EDTA-NO by sulfide went up along with the decrease of the pH values and rise of the reaction temperature. Besides, the energy of activation (E-a) and the entropy of activation (Delta S-double dagger) were 31.125 kJ mol(-1) and -67.6144 J K-1 mol(-1), respectively. Apart from the investigation into the abiotic role of sulfide on the BioDeNO(x) process, this paper also provided a hint on an integrated chemical and biological process for simultaneous desulfurization and denitrification.