The present study has been undertaken to assess integrated algal harvesting in sewage wastewater for biodiesel feedstock. Chlorella minutissirna microalgae are grown in wastewater from local hotel sewage drain and produced biomass is used to produce biodiesel. C. minutissima effectively removes the wastewater nutrients nitrogen and phosphorus with the 99.19% and 96% removal respectively. Content of oil in produced algal biomass is drastically reduced i.e. 4% in response to 57% of total dry biomass reported in literature. The extracted oil mainly consists of small chain fatty acids (C-16:0, C-18:1, C-18:2, C-18:3). Transesterification reaction shows that maximum fatty acid methyl ester (FAMEs) concentration is converted from free fatty acids of oil during 1-2 h at 65 degrees C.