Journal of Bioscience and Bioengineering, Vol.116, No.3, 298-301, 2013
Intracellular protein cyclization catalyzed by exogenously transduced Streptococcus pyogenes sortase A
Streptococcus pyogenes sortase A (SpSrtA) was transduced into mammalian cells. Then SpSrtA-mediated intracellular circularization of a model protein was confirmed in a time and dose-dependent manner by Western blotting analysis. Direct transduction of SpSrtA is expected to be a strong tool for more conditional specific protein modification in mammalian cells. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.