순환 유도관형 삼상 유동층 생물반응기에서 고정화 Pseudomonas putida에 의한 페놀처리

박정권; 염경호

Journal of the Korean Industrial and Engineering Chemistry, Vol.6, No.3, 501-509, June, 1995

Export Citation

순환 유도관형 삼상 유동층 생물반응기에서 고정화 Pseudomonas putida에 의한 페놀처리

Phenol Degradation by Immobilized Pseudomonas putida in Draft-tube Three-phase Fluidized-bed Bioreactor

박정권, 염경호

초록

순환 유도관형 삼상 유동층 반응기의 미생물막 반응기로서의 유용성과 조작안정성을 규명하기 위해 활성탄에 고정화시킨 Pseudomonas putida 혼합 미생물을 이용하여 페놀함유 폐수의 생물학적 처리실험을 수행하였다. 이 결과 페놀 부하량 5g/ℓ·day 까지는 95% 이상의 높은 페놀처리율을 나타내었으나, 그 이상의 페놀 부하량에서는 미생물막 밀도의 증가에 따른 기질인 페놀의 미생물막내로의 확산 감소로 인해 페놀처리율이 점차 감소하였다. 순환 유도관형과 기존의 단순형 삼상 유동층 생물반응기에서의 미생물막 밀도변화에 따른 페놀처리율을 비교한 결과, 순환 유도관형 삼상 유동층 생물반응기는 미생물막의 과코성장을 억제할 수 있으며 넓은 페놀 부하량 범위에 대해 운전이 가능하여 단순형 삼상 유동층 생물반응기보다 조작 안정성이 우수하였다.

The usability and operational stability of the draft-tube three-phase fluidized-bed reactor(DTFR ) as a biofilm reactor are analyzed using the experimental results of phenol biodegradation by Pseudomonas putida mixed cultures immobilized on the activated carbon. The phenol conversions of greater than 95 % are obtained when the phenol loading is below 5g/L·day. But the phenol conversions decrease gradually with increasing the phenol loading beyond the value of 5g/L·day due to the augmentation of biofilm density which reduces the phenol diffusion rate into the biofilm. The operational stability of DTFR is more excellent than the conventional three-phase fluidized-bed reactor(CTFR), because DTFR restrains more efficiently the heavy growth of biofilm and could be operated for the more wider ranges of phenol loading.

[References]

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[1] Klein JA, Lee DD, Biotechnol. Bioeng.(8), 379 (1978)

[2] Lanouette KH, Chem. Eng., Deskbook Issue, Oct.(17), 99 (1977)

[3] Hill GA, Robinson CW, Biotechnol. Bioeng., 17, 1599 (1975)

[4] Hsu HW, Biotechnol. Bioeng.(8), 1 (1978)

[5] 김우식, 염경호, 김응식, 한국산업미생물학회지, 13(1), 33 (1985)

[6] Livingston AG, Chase HA, AIChE J., 35, 1980 (1989)

[7] Tang WT, Fan LS, AIChE J., 33, 239 (1987)

[8] Livingston AG, Biotechnol. Bioeng., 38, 260 (1991)

[9] Park YH, "Catalogue of Strains," 2nd Ed., Korea Collection for Type Culture (KCTC), Seoul (1989)

[10] "Standard Methods for the Examination of Water and Wastewater," 14th Ed., Amer. Pub. Health Ass. (APHA), New York (1975)

[11] Haldane JSB, "Enzymes," Longmans, London (1930)

[12] Fan LS, Fujie K, Long TR, Tang WT, Biotechnol. Bioeng., 30, 498 (1987)

[13] Lee DD, Scott CD, Hancher CW, J. WPCF, 51, 974 (1979)

[14] Holladay DW, Hancher CW, Scott CD, Chilcote DD, J. WPCF, 50, 2573 (1978)