The synthesis of four amphiphilic thieno[3,4-c]pyrrole-4,6-dione (TPD)-based alternating copolymers and their behavior at the air-water interface are reported. Homogeneous and stable monolayers of TPD-based copolymers were prepared. Brewster angle microscopy (BAM) was utilized to characterize the morphology and topography of these Langmuir films, UV-vis absorption spectroscopy as well as atomic force microscopy has revealed a regular transfer of some copolymers on glass substrates. It was possible to obtain homogeneous Langmuir-Blodgett films of up to 30 layers. Infrared dichroic measurements revealed an edge-on orientation. These Langmuir-Blodgett films made of conjugated polymers are therefore good candidates for organic field-effect transistors (OFETs).