TiO2 photocatalysts co-doped with different content of Ag and N were prepared by sol-gel method combined with microwave chemical method. The samples were analyzed by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscope (TEM), ultraviolet-visible diffuse reflectance spectrum (UV-vis) and photo-luminescence emission spectrum (PL). The photocatalytic activity was investigated by photocatalytic degradation of methylene blue (MB) under irradiation of fluorescent lamp. The results indicate that Ag and N co-doping can restrain the increase of grain size, broaden the absorption spectrum to visible light region, and inhibit the recombination of the photo-generated electron-hole pairs. Moreover, the photocatalytic activity of Ag-N-TiO2 in MB degradation is remarkable improved. The degradation rate of the sample with Ag:TiO2 = 0.05 at%, N:TiO2 = 18.50 wt% in 5 h is 93.44%, which is much higher than that of Degussa P25 (39.40%). (C) 2013 Elsevier Ltd. All rights reserved.