Biosurfactants are produced by microorganisms, especially those of the genus Pseudomonas. This study is concerned with the recovery of rhamnolipids produced by Pseudomonas aeruginosa P029-GVIIA, using molasses as substrate. A central compound design 2(3) in triplicate at the central point was used to evaluate, the influence of the centrifugation time, the agitation speed, and the pH on the amount of rhamnolipids precipitated by HCl (2N). A 2(4) factorial design in triplicate at the central point was used to investigate the influence of the pH (3-10), temperature (30 to 50 degrees C), the concentration of carbon (1-3% w/v), and the agitation speed (100-200rpm) on the adsorption of rhamnolipids to activated carbon. The tests showed that the adsorption is governed particularly by the pH and the temperature, as well as by the temperaturexpH interaction. A pseudo-first order kinetic model successfully fitted the data, showing that the adsorbent had the ability to adsorb approximately 17.16mg of rhamnolipids/gram of activated carbon.