Quantitative analysis of microbial community structure in two-phase anaerobic digesters treating food wastewater

Woong Kim; Byung-Gon Ryu; Sungwhan Kim; Sung-Woon Heo; Donghyun Kim; Jungmin Kim; Haechan Jo; Jong-Hee Kwon; Ji-Won Yang

Korean Journal of Chemical Engineering, Vol.31, No.3, 381-385, March, 2014

DOI10.1007/s11814-014-0019-1 Export Citation

Quantitative analysis of microbial community structure in two-phase anaerobic digesters treating food wastewater

Woong Kim¹, Byung-Gon Ryu², Sungwhan Kim¹, Sung-Woon Heo¹, Donghyun Kim¹, Jungmin Kim¹, Haechan Jo³, Jong-Hee Kwon¹, and Ji-Won Yang^{1, 4, †}

¹Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea
²Environmental and Energy Program, KAIST, 291, Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea
³Yangseo High School, 31-12, Sangchon-gil, Yangseo-myeon, Yangpyeong-gun, Gyeonggy 476-824, Korea
⁴Advanced Biomass R&D Center, KAIST, 291, Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea

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An acidogenic reactor with a 0.5-L working volume and a methanogenic digester with a 5-L of working volume were operated for 150 days on a continuous mode to investigate the structure of a microbial community during food wastewater treatment. During the steady state of anaerobic digestion, volatile solids (VS) removal efficiency in the pilot plant was approximately 65%. The bacterial population was higher than any other methanogens detected during the entire anaerobic process and treatment of raw food wastewater. Methanomicrobiales (MMB), Methanosarcinales (MSL), and Methanobacteriales (MBT) were detected during digestion. The methanogenic population present in the acidogenic reactor was directly affected by the archaeal community in raw food wastewater. However, the shift of microbial community in the methanogenic digester was relatively gradual. The performance of methanogenic digester might be more related to the change of microbial metabolism affected by the physicochemical properties of the input substrate.

Keywords:Food Wastewater;Anaerobic Digestion;Methanobacteriales;Methanomicrobiales;Methanosarcinales

[References]

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Speece RE, Archae Press, Nashville, TN (1996)
Yu YS, Lee CS, Kim J, Hwang SK, Biotechnol. Bioeng., 89(6), 670 (2005)
Hori T, Haruta S, Ueno Y, Ishii M, Igarashi Y, Appl. Environ. Microbiol., 72, 1623 (2006)
Curtis TP, Sloan WT, Curr. Opin. Microb., 7, 221 (2004)
Kim W, Shin S, Cho K, Han G, Hwang S, Bioprocess. Biosyst. Eng., 1 (2013)
Fernandez AS, Hashsham SA, Dollhopf SL, Raskin L, Glagoleva O, Dazzo FB, Hickey RF, Criddle CS, Tiedje JM, Appl. Environ. Microbiol., 66, 4058 (2000)
APHA-AWWA-WEF, American Public Health Association, Washinton, DC (2005)
Yu Y, Kim J, Hwang S, Biotechnol. Bioeng., 93(3), 424 (2006)
Obata K, Segawa O, Yakabe M, Ishida Y, Kuroita T, Ikeda K, Kawakami B, Kawamura Y, Yohda M, Matsunaga T, Tajima H, J. Biosci. Bioeng., 91(5), 500 (2001)
Yu Y, Lee C, Hwang S, Water Sci. Technol., 52, 85 (2005)
Muyzer G, De Waal EC, Uitterlinden AG, Appl. Environ. Microbiol., 59, 695 (1993)
Metje M, Frenzel P, Appl. Environ. Microbiol., 71, 8191 (2005)
Garcia JL, Patel BKC, Ollivier B, Anaerobe., 6, 205 (2000)
Ahn JH, Do TH, Kim SD, Hwang S, Biochem. Eng. J., 30, 33 (2006)
Boone DR, Castenholz RW, Springer, New York (2001)
Anderson IJ, Sieprawska-Lupa M, Goltsman E, Lapidus A, Copeland A, Glavina DRT, Tice H, Dalin E, Barry K, Pitluck S, Hauser L, Land M, Lucas S, Richardson P, Whitman WB, Kyrpides NC, Stand Genomic Sci., 1, 197 (2009)
Stams AJM, Plugge CM, Nat. Rev. Micro., 7, 568 (2009)
Peng J, Lu Z, Rui J, Lu Y, Appl. Environ. Microbiol., 74, 2894 (2008)
Blaut M, Antonie van Leeuwenhoek, 66, 187 (1994)
Simeonov I, Karakashev D In: Preprints 7th Vienna Internat. Conf. on Math. Modelling (MATHMOD) on CD (2012)
Kim W, Lee S, Shin SG, Lee C, Hwang K, Hwang S, Water Res., 44, 4900 (2010)

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[2] Speece RE, Archae Press, Nashville, TN (1996)

[3] Yu YS, Lee CS, Kim J, Hwang SK, Biotechnol. Bioeng., 89(6), 670 (2005)

[4] Hori T, Haruta S, Ueno Y, Ishii M, Igarashi Y, Appl. Environ. Microbiol., 72, 1623 (2006)

[5] Curtis TP, Sloan WT, Curr. Opin. Microb., 7, 221 (2004)

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[7] Fernandez AS, Hashsham SA, Dollhopf SL, Raskin L, Glagoleva O, Dazzo FB, Hickey RF, Criddle CS, Tiedje JM, Appl. Environ. Microbiol., 66, 4058 (2000)

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[9] Yu Y, Kim J, Hwang S, Biotechnol. Bioeng., 93(3), 424 (2006)

[10] Obata K, Segawa O, Yakabe M, Ishida Y, Kuroita T, Ikeda K, Kawakami B, Kawamura Y, Yohda M, Matsunaga T, Tajima H, J. Biosci. Bioeng., 91(5), 500 (2001)

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[12] Muyzer G, De Waal EC, Uitterlinden AG, Appl. Environ. Microbiol., 59, 695 (1993)

[13] Metje M, Frenzel P, Appl. Environ. Microbiol., 71, 8191 (2005)

[14] Garcia JL, Patel BKC, Ollivier B, Anaerobe., 6, 205 (2000)

[15] Ahn JH, Do TH, Kim SD, Hwang S, Biochem. Eng. J., 30, 33 (2006)

[16] Boone DR, Castenholz RW, Springer, New York (2001)

[17] Anderson IJ, Sieprawska-Lupa M, Goltsman E, Lapidus A, Copeland A, Glavina DRT, Tice H, Dalin E, Barry K, Pitluck S, Hauser L, Land M, Lucas S, Richardson P, Whitman WB, Kyrpides NC, Stand Genomic Sci., 1, 197 (2009)

[18] Stams AJM, Plugge CM, Nat. Rev. Micro., 7, 568 (2009)

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[21] Simeonov I, Karakashev D In: Preprints 7th Vienna Internat. Conf. on Math. Modelling (MATHMOD) on CD (2012)

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