화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Journal of Chemical Engineering, Vol.31, No.4, 684-691, April, 2014
DOI10.1007/s11814-013-0269-3  Export Citation
Modeling and simulation of breakthrough curves during purification of two chitosanases from Metarhizium anisopliae using ion-exchange with expanded bed adsorption chromatography
Sergio Carvalho de Santana1, 2, Raimundo Cosme da Silva Filho2, Jorge dos Santos Cavalcanti2, Jackson Araujo de Oliveira2, Gorete Ribeiro de Macedo2, Francine Ferreira Padilha3, 4, and Everaldo Silvino dos Santos2, †
  • 1Northeast Biotechnology Network (RENORBIO), Technology and Research Institute (ITP), Tiradentes University (UNIT), Biomaterial Laboratory (LBMAT), Aracaju/SE, Av. Murilo Dantas 300,Farolândia, Aracaju/SE, 49032-490, Brazil
  • 2Biochemical Engineering Laboratory, Department of Chemical Engineering, Federal University of Rio Grande do Norte (UFRN), Natal/RN, 59072-970, Brazil
  • 3Northeast Biotechnology Network (RENORBIO), Tiradentes University (UNIT), Aracaju/SE, Brazil
  • 4Technology and Research Institute (ITP), Biomaterial Laboratory (LBMAT), Av. Murilo Dantas 300,Farolândia, Aracaju/SE, 49032-490, Brazil
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A mathematical model was developed to predict breakthrough curves during purification of the two chitosanases from Metarhizium anisopliae by expanded bed adsorption, taking into account the axial dispersion of liquid and using Streamline DEAE and SP XL adsorbents, anion and cation exchange resins, respectively. All the experiments were performed without clarification (with cells) aiming at the reduction of unit operations in future projects of separation processes, thereby reducing capital and operating costs. Chitosanases are enzymes that hydrolyze the carbohydrate chitosan, resulting in oligosaccharides that have many remarkable biological activities, such as anti-cancer, anti-HIV and antioxidant activities. The two adsorbents had similar performance in relation to hydrodynamics and mass transfer. The results of the parametric sensitivity analysis agree with the literature, and the model was validated with an average high degree of fit (94.68%) between simulated and experimental data obtained in this work.
Keywords:Modeling;Expanded Bed Adsorption;Purification;Chitosanase;Metarhizium anisopliae
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