화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Chemical Engineering Research, Vol.52, No.3, 402-406, June, 2014
Export Citation
목질계 농부산물을 이용한 고체발효에서 발효조건 최적화를 통한 구연산 생산 증대
Optimization of Fermentation Conditions for the Production of Citric Acid by Aspergillus niger NRRL 567 Grown on Agricultural by Products
김진우†
  • 맥길대학교 바이오시스템공학과, 몬트리올, 캐나다, H9X 3V9
Jin-Woo Kim†
  • Department of Biosystems Engineering, McGill University, Montreal, Canada, H9X 3V9, Korea
E-mail:
초록
본 실험에서는 농부산물인 밀짚을 고체배지로 사용하여 Aspergillus niger NRRL 567에서 구연산 생산 시, 발효조건과 첨가제가 구연산 생산에 미치는 영향을 단일변수(one-factor-at-a-time) 최적화를 이용하여 주요 인자의 순차적 최적화를 수행하였다. 발효 72시간에서 온도, 수분함량, 입자크기, pH와 첨가제 농도를 최적화했을 때, 각각 30 oC, 70%, 0.5~1.0 mm, pH 5.5와 4% 메탄올 첨가조건에서 최대 구연산 생산인 206.0 g/kg 건조중량 (DM)을 확인할 수 있었다. 이는 최적화 이전 구연산 최고 생산인 74.5 g/kg DM 대비 177% 증가한 결과이다. 최적화 실험에서 도출된 조건을 밀짚, 옥수수대와 피트모스(peat moss)에 적용하여 고체발효를 수행하였을 때, 발효 120시간에서 각각 231.8, 213.8, 240.2 g/kg DM 구연산 생산을 확보하였다. 본 실험 결과는 밀짚과 옥수수대 등의 목질계 농부산물을 이용한 구연산 생산 시, 고체발효법이 기존의 액체발효법의 대체가 가능함을 시사하였다.
The present study was carried out to evaluate the potential of lignocellulosic byproducts for the production of citric acid through solid-state fermentation by Aspergillus niger NRRL 567. A sequential optimization based on onefactor-at-a-time method was applied to optimize fermentation conditions and media constituents. The results obtained from the optimization indicated that 30 oC, 70% moisture content, 0.5~1.0 mm particle size, pH 5.5 and 4% methanol were found to be the optimum condition at 72 hr fermentation. The application the optimization resulted in an improvement of maximum citric acid production from 74.5 to 206.0 g/kg dry material (DM) from wheat straw. The optimal condition was used to produce citric acid from A. niger grown on different lignocellulosic byproducts, including wheat straw, corn stover and peat moss. A. niger produced the highest citric acid levels of 231.8, 213.8 and 240.2 g/kg DM at 120 hr fermentation, respectively.
Keywords:Solid State Fermentation;Citric Acid;Rice Straw;Optimization;Agricultural by Products
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