Effect of reaction parameters on conversion of krill (Euphausia superba) oil by immobilized lipase ethanolysis

Hye-youn Lee; A.S.M. Tanbirul Haque; Seon-Bong Kim; Yang-Bong Lee; Byung-Soo Chun

Journal of Industrial and Engineering Chemistry, Vol.20, No.3, 1097-1102, May, 2014

DOI10.1016/j.jiec.2013.06.047 Export Citation

Effect of reaction parameters on conversion of krill (Euphausia superba) oil by immobilized lipase ethanolysis

Hye-youn Lee, A.S.M. Tanbirul Haque, Seon-Bong Kim, Yang-Bong Lee, and Byung-Soo Chun^†

Department of Food Science and Technology, Pukyong National University, 45 Yongso-ro, Nam-Gu, Busan 608-737, Republic of Korea

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Monoglyceride and diglyceride were produced by performing ethanolysis of krill oil with immobilized lipase and the influence of various parameters on the enzymatic ethanolysis was assessed. As an immobilized lipase, lipozyme TL-IM (thermonuces lanuginose) was used. Ethanolysis was done in nonpressurized and pressurized system to compare the reaction rate and yield. The optimal condition was found at 2.0 of ethanol mole ratio, temperature of 60 ℃, lipases amount of 5 wt% in non-pressurized system. At pressurized system the optimal temperature and pressure was found at 50 ℃ and 10 MPa. However, at 50 ℃ monoglyceride was higher in pressurized system than in non-pressurized system.

Keywords:Monoglyceride;Diglyceride;Ethanolysis;Immobilized lipase;Krill oil

[References]

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[Referenced By]

[1] Zhao HZ, Lu ZX, Bie XM, Lu FX, Liu ZM, J. Food Eng., 78(1), 41 (2007)

[2] Dliveira J, Valdimir O, J. Supercrit. Fluids, 19, 141 (2001)

[3] Maki KC, Davidson MH, Tsushima R, Matsuo N, Tokimitsu I, Umporowicz DN, Dicklin MR, Foster GS, Ingram KA, Anderson BD, Forst SD, Bell M, Am. J. Clinical Nutrition, 76, 1230 (2002)

[4] Kwon SJ, Han JJ, Rhee JS, Enzyme Microb. Technol., 7, 700 (1995)

[5] Watanabe T, Shimizu M, Sugiura M, Sato M, Kohori J, Yamada N, Nakanishi K, J. Am. Chem. Soc., 80, 1201 (2003)

[6] Yasunaga K, Glinsmann WH, Seo Y, Katsuragi Y, Kobayashi B, Flickinger E, Kennepohl T, Yasukawa JF, Borzelleca, Food Chem. Toxicol., 42, 1419 (2004)

[7] Shaw JF, Wang DL, Wang YJ, Enzyme Microb. Technol., 13, 544 (1991)

[8] Yesiloglu Y, J. Am. Chem. Soc., 81, 157 (2004)

[9] Lee KW, Hailan C, Yinhua J, Kim YW, Chung KW, Korean J. Chem. Eng., 25(3), 474 (2008)

[10] Erickson JC, Schyns P, Cooney CL, AIChE J., 36, 299 (1990)

[11] Steytler DC, Moulson PS, Reynolds J, Enzyme Microb. Technol., 13, 221 (1992)

[12] Kuhn G, Marangoni M, Freire DMG, Soares VF, de Godoy MG, de Castro AM, Di Luccio M, Treichel H, Mazutti MA, Oliveira D, Oliveira JV, J. Chem. Technol. Biotechnol., 85(6), 839 (2010)

[13] Kaewthong W, Sirisansaneeyakul S, Prasertsan P, Kittikun AH, Process Biochem., 40, 1525 (2005)

[14] Holcapek M, Janadera P, Fischer J, Prokes B, J. Chromatogr. A, 858, 13 (1999)

[15] Kulkarni MG, Dalai AK, Bakhshi NN, Bioresour. Technol., 10, 2027 (2007)

[16] Simada Y, Watanabe Y, Sugihara A, Tominaga Y, J. Mol. Catal. B-Enzym., 17, 133 (2002)

[17] Hasan F, Shah AA, Hameed A, Enzyme Microb. Technol., 39(2), 235 (2006)

[18] Kwon CH, Lee JH, Kim SW, Kang JW, J. Microbiol. Biotechnol., 19, 1596 (2009)