Biosynthesis of silver nanoparticles from the culture supernatant of Bacillus marisflavi and their potential antibacterial activity

Kevin John Pulikotil Anthony; Muralidharan Murugan; Sangiliyandi Gurunathan

Journal of Industrial and Engineering Chemistry, Vol.20, No.4, 1505-1510, July, 2014

DOI10.1016/j.jiec.2013.07.039 Export Citation

Biosynthesis of silver nanoparticles from the culture supernatant of Bacillus marisflavi and their potential antibacterial activity

Kevin John Pulikotil Anthony¹, Muralidharan Murugan¹, and Sangiliyandi Gurunathan^{1, 2, †}

¹GS Centre for Life Sciences, 13 Lords Avenue, Sundarapuram, Coimbatore 641024, Tamilnadu, India
²Dept. of Animal Biotechnology, Konkuk University, 1 Hwayang-Dong, Gwangin-gu, Seoul 143-701, Republic of Korea

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Silver nanoparticles (AgNPs) were prepared using culture supernatant of Bacillus marisflavi, a novel strain isolated from agricultural wastes. The formation of the AgNPs was confirmed using various analytical techniques such as UV-vis absorption spectroscopy, dynamic light scattering (DLS) and transmission electron microscopy (TEM). The antibacterial effect of synthesized nanoparticles was investigated against Pseudomonas aeruginosa. Furthermore, the mechanism of action of cell death of P. aeruginosa was confirmed by reactive oxygen species (ROS) generation in AgNPs treated cells and also our results clearly indicate that AgNPs could induce generation of ROS by dose dependent manner.

Keywords:AgNPs;B. marisflavi;UV-visible absorption Spectrum;ROS generation;Transmission electron microscopy

[References]

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[Referenced By]

[1] Mazur M, Electrochem. Commun., 6, 400 (2004)

[2] Rosemary MJ, Pradeep T, Colloids Surf. A: Physicochem. Eng. Asp., 268, 81 (2003)

[3] Maillard M, Giorgio S, Pileni MP, Adv. Mater., 14(15), 1084 (2002)

[4] Pillai ZS, Kamat PV, J. Phys. Chem. B, 108(3), 945 (2004)

[5] Patel K, Kapoor S, Dave DP, Murherjee T, J. Chem. Sci., 117, 53 (2005)

[6] Salkar RA, Jeevanandam P, Aruna ST, Koltypin Y, Gedanken A, J. Mater. Chem., 9, 1333 (1999)

[7] Zhu JJ, Liao XH, Zhao XN, Hen HY, Mater. Lett., 49, 91 (2001)

[8] Shahverdi AR, Fakhimi A, Shahverdi HR, Minaian S, J. Nanomedicine, 3, 168 (2007)

[9] Kalimuthu K, Babu RS, Venkataraman D, Bilal M, Gurunathan S, Colloids Surf. B: Biointerfaces, 65, 150 (2008)

[10] Gurunathan S, Kalishwaralal K, Vaidyanathan R, Deepak V, Pandian SR, Jeyaraj M, Hariharan N, Eom SH, Colloids Surf. B: Biointerfaces, 74, 328 (2009)

[11] Gupta A, Maynes M, Silver S, Appl. Environ. Microbiol., 64, 5042 (1998)

[12] Duran N, Marcato PD, De Souza GIH, Alves LO, Esposito EE, Biomedical Nanotechnology, 3, 203 (2007)

[13] Pal S, Tak YK, Song JM, Appl. Environ. Microbiol., 27, 1712 (2007)

[14] Klaus-Joerger T, Joerger R, Olsson E, Granqvist CG, Trends in Biotechnology, 19, 15 (2001)

[15] Schultz S, Smith DR, Mock JJ, Schultz DA, Proceedings of the National Academy, 97, 996 (2000)

[16] Perugini P, Simeoni S, Scalia S, Genta I, Modena T, Conti B, Pavanetto F, Int. J. Pharmaceutics, 246, 37 (2002)

[17] Jin S, Ye KM, Biotechnol. Prog., 23(1), 32 (2007)

[18] Czupryna J, Tsourkas A, Cancer Biology Therapy, 5, 1691 (2006)

[19] Prow T, Grebe R, Merges C, Smith J, McLeod S, Leary J, Lutty G, Int. J. Nanomedicine, 12, 616 (2006)

[20] Kalishwaralal K, BarathManiKanth SS, Pandian SRK, Deepak V, Gurunathan S, Colloids Surf. B: Biointerfaces, 79, 334 (2010)

[21] Whitesides GM, Nat. Biotechnol., 21, 1161 (2003)

[22] Fortin D, Beveridge TJ, From Biology to Biotechnology and Medical Applications, Wiley-VCH, Weinheim, Germany, 2000.

[23] Klaus T, Joerger R, Olsson E, Ranqvist CG, Proceedings of the National Academy of Sciences 96 (1999) 13611.

[24] Duran N, Marcato PD, Alves OL, Souza GI, Esposito E, Nanobiotechnology, 3, 8 (2005)

[25] Parikh RYS, Singh S, Prasad BLV, Patole MS, Sastry M, Shouche YS, Chembiochem, 9, 1415 (2008)

[26] Pugazhenthiran N, Anandan S, Kathiravan G, Prakash NKU, Crawford S, Ashokkumar N, Nanoparticle Research, 11, 1811 (2009)

[27] Kalishwaralal K, Deepak V, Ramkumarpandian S, Nellaiah H, Sangiliyandi G, Mater. Lett., 62, 4411 (2008)

[28] Ahmad A, Mukherjee P, Senapati S, Mandal D, Khan MI, Kumar R, Sastry M, Colloids Surf. B: Biointerfaces, 28, 313 (2003)

[29] Sastry M, Patil V, Sainkar SR, J. Phys. Chem. B, 102(8), 1404 (1998)

[30] Sastry M, Mayya KS, Bandyopadhyay K, Colloids Surf. A: Physicochem. Eng. Asp., 127, 221 (1997)

[31] Jain D, Sumitha K, Rohit J, Garima S, Kothari SL, J. Exp. Biol., 48, 1152 (2010)

[32] Nel A, Xia T, Madler L, Li N, Science, 311, 622 (2006)

[33] Sondi I, Salopek-Sondi B, J. Colloid Interface Sci., 275(1), 177 (2004)

[34] Su HL, Chou CC, Hung DJ, Lin SH, Pao IC, Lin JH, Huang FL, Dong RX, Lin JJJ, Biomaterials, 30, 5979 (2009)

[35] Guzman MG, Dille J, Godet S, Int. J. Chem. Biomol. Eng., 2(3), 2009 (2009)

[36] Carlson C, Hussain SM, Schrand AM, Braydich-Stolle LK, Hess KL, Jones RL, Schlager JJ, J. Phys. Chem. B, 112(43), 13608 (2008)

[37] Guzman GM, Dille J, Godet S, J. Chem. Biol. Eng., 2, 3 (2009)

[38] Cho KH, Park JE, Osaka T, Park SG, Electrochim. Acta, 51(5), 956 (2005)

[39] Raffi M, Hussain F, Bhatti TM, Akhter JI, Hameed A, Hasan MM, J. Mater. Sci. Technol., 24, 192 (2008)

[40] Panacek A, Kvitek L, Prucek R, Kolar M, Vecerova R, Pizurova N, Sharma VK, Nevecna T, Zboril R, J. Phys. Chem. B, 110(33), 16248 (2006)

[41] Jain J, Arora S, Rajwade MJ, Omray P, Khandelwal SS, Kishore MK, Paknikar M, Mol. Pharm., 6, 1388 (2009)