Recently, in all over the world, nanotechnology plays a major role in various applications. Most of the researchers focused their work on bimetallic nanoparticles due to their several modes or mechanisms of synthesis such as chemical, physical, and biosynthesis methods. These nanoparticles are of great interest due to their enormous applications and catalytic activities. Currently, syntheses of bimetallic nanoparticles using different sources of natural products are focused due to their advantage of being nontoxic to human and environment. To our knowledge, there is no report on the review of bimetallic nanoparticles and their medicinal applications. Taking this fact into account, we discussed the various synthesizing methods of bimetallic nanoparticles and their application related to biology.