A novel fructosyltransferase (AoFT) capable of synthesizing sucrose 6-acetate (S6A) from sucrose and glucose 6-acetate has been purified to homogeneity from Aspergillus oryzae ZZ-01. Its molecular mass was similar to 50 kDa by SDS-PAGE; optimal activity was at 45 degrees C and it was stable from pH 4.5 to 7.5 with an optimum pH of 6. Mg2+, K+ (5 mM), propanol, toluene (50 %, v/v), Tween 20 or Triton X-100 (1 %, w/v) increased the transfructosylation activity by 20, 17, 17, 10, 25 and 20 %, respectively. An overall conversion of 32 % was achieved under optimal conditions over 24 h. This is the first report that the purified and characterized the fructosyltransferase from Aspergillus capable of synthesis of S6A from sucrose and glucose 6-acetate.