Making an artificial iris with an aesthetically acceptable color is an important aspect of ocular rehabilitation. This work evaluated the influence of different disinfecting solutions on changes to the color of artificial irises used in ocular prostheses. Fifty samples simulating ocular prostheses were produced with cobalt blue artificial irises and divided (n = 10) according to the disinfectant used: neutral soap, Opti-free, Efferdent, 1% hypochlorite, and 4% chlorhexidine. The samples were disinfected for 120 days and subjected to a color readings by spectrophotometry, using the CIE L*a*b* system, before the disinfection period (B), after 60 days of disinfectant exposure (T-1), and after 120 days of disinfectant exposure (T-2). Color differences (E) were calculated for the intervals between T-1 and B (T1B), and between T-2 and B (T2B). The data were evaluated by analysis of variance and the Tukey Honestly Significantly Different ( = 0.05). All disinfectant groups exhibited color changes. The mean color change observed for all groups overall during T2B (E = 3.51) was significantly greater than that observed during T1B (E = 2.10). All groups exhibited greater color change for the b* values when compared to the a* and L* values. There were no significant differences between the disinfectant groups. It can be concluded that the time period of disinfection and storage significantly affected the stability of artificial iris color, independent of the disinfectant used. (c) 2012 Wiley Periodicals, Inc. Col Res Appl, 39, 56-62, 2014