Cyclotron resonance has been investigated on single-layer large-area graphene which was synthesized by chemical vapor deposition. Absorption peaks of the cyclotron resonance are clearly observed on the graphene sample and these peak energies exhibit root B-dependence in terms of an unequally spaced Landau Level structure in Dirac fermions. We find significant substrate dependence of the band velocity E. The derived E of the sample on a GaAs substrate is enhanced by about 10% from that on a glass substrate which almost coincides with that of the multi-layer epitaxial graphene, indicating that the E is strongly affected by the condition of the substrate. (C) 2013 Elsevier B.V. All rights reserved.