Ethanol fermentation from xylose by metabolically engineered strains of Kluyveromyces marxianus

Goshima T; Negi K; Tsuji M; Inoue H; Yano S; Hoshino T; Matsushika A

Journal of Bioscience and Bioengineering, Vol.116, No.5, 551-554, 2013

DOI10.1016/j.jbiosc.2013.05.010 Export Citation

Ethanol fermentation from xylose by metabolically engineered strains of Kluyveromyces marxianus

Goshima T, Negi K, Tsuji M, Inoue H, Yano S, Hoshino T, Matsushika A

We constructed a xylose-fermenting recombinant strain of thermotolerant yeast Kluyveromyces marxianus, DMB3-7. Both xylose consumption and ethanol production were remarkably increased in DMB3-7 compared to the control strain at 30 degrees C. Furthermore, DMB3-7 produced ethanol from xylose at both 42 degrees C and 45 degrees C, above which xylose metabolic activity decreased.

Keywords:Thermotolerant yeast;Bioethanol;Xylose;Xylose reductase;Xylitol dehydrogenase;Xylulokinase