Application of fungal laccase fused with cellulose-binding domain to develop low-lignin rice plants

Furukawa T; Sawaguchi C; Watanabe A; Takahashi M; Nigorikawa M; Furukawa K; Iimura Y; Kajita S; Oguchi T; Ito Y; Sonoki T

Journal of Bioscience and Bioengineering, Vol.116, No.5, 616-619, 2013

DOI10.1016/j.jbiosc.2013.05.007 Export Citation

Application of fungal laccase fused with cellulose-binding domain to develop low-lignin rice plants

Furukawa T, Sawaguchi C, Watanabe A, Takahashi M, Nigorikawa M, Furukawa K, Iimura Y, Kajita S, Oguchi T, Ito Y, Sonoki T

We observed a reduction of lignin content linked to the expression of fungal laccase in rice plants. The lignin content of L-4, which showed the highest LAC activity among transgenic lines produced, was lower than that of the control line. However, this change was not reflected to the saccharification efficiency. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.

Keywords:Fusion protein;Laccase;Trametes versicolor;Cellulose-binding domain;Ouzo sativa L.