Nanofluids are simply the dispersion of nanometer-sized particles in different fluids. Graphene oxide nanosheets (GONs) were prepared by exfoliating the graphite oxide. The GONs were investigated using Fourier transform-infrared spectroscopy, Raman spectroscopy, XRD analysis, high-resolution emission electron microscopy, transmission electron microscopy, and UV-visible spectroscopy. GONs/glycerol-water-based nanofluid was prepared by the two-step method. The stability of the nanofluid was investigated with respect to time. Thermal and electrical conductivity of the prepared nanofluid was examined with different temperatures (25-45 A degrees C) and weight fractions (0.02-0.1 wt%). The nanofluid is found to be stable for more than 5 months. The results showed an enhancement in thermal conductivity of about 4.5 % at 25 A degrees C with a weight fraction of 0.02 %. The improvement was up to 11.7 % with a weight fraction of 0.1 wt% at 45 A degrees C. The electrical conductivity was increased with increasing the weight fraction and temperature. The improvement in electrical conductivity was about 5890 % at 25 A degrees C and 0.1 wt%.