In this paper, Siberian apricot (Prunus sibirica L.) seed kernel oil was investigated for the first time as a promising non-conventional feedstock for preparation of biodiesel. Siberian apricot seed kernel has high oil content (50.18 +/- 3.92%), and the oil has low acid value (0.46 mg g(-1)) and low water content (0.17%). The fatty acid composition of the Siberian apricot seed kernel oil includes a high percentage of oleic acid (65.23 +/- 4.97%) and linoleic acid (28.92 +/- 4.62%). The measured fuel properties of the Siberian apricot biodiesel, except cetane number and oxidative stability, were conformed to EN 14214-08, ASTM D6751-10 and GB/T 20828-07 standards, especially the cold flow properties were excellent (Cold filter plugging point -14 degrees C). The addition of 500 ppm tert-butylhydroquinone (TBHQ) resulted in a higher induction period (7.7 h) compliant with all the three biodiesel standards. (C) 2012 Elsevier Ltd. All rights reserved.