In order to evaluate the leaching of cinnabar, a chemobiological reactor system with Acidithiobacillus ferrooxidans for cinnabar dissolution was investigated. The results demonstrated cinnabar dissolution had relation to bioprocess of A. ferrooxidans and iron concentration tightly. The optimal dilution rate and iron concentration were 0.4/h and 2-3 g/L in chemobiological reactor. The process may be contributed to the indirect catalyzing of ferric iron generated with A. ferrooxidans and direct adherence oxidation function. This research shows the new microbiological technique may be a feasible and economical method in application. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.