Novel expansins, non-catalytic proteins which induce weakening of the rigid cellulose structure, have been identified in this study. A pipeline of bioinformatics was implemented for sequence and structure-based prediction of putative bacterial expansin-like group x family from NR databases. All putative expansins had no detectable activity against cellulosic and hemicellulosic substrates but showed varying degrees of synergy (2.0-7.6 folds) with the commercial Trichoderma reesei cellulase (Celluclast T 1.5 L) on degradation of filter paper in order of BpEX approximate to CmEX > MaEX > PcEX > SaEX. A mixture design with full cubic model predicted optimal formulation comprising Celluclast (TM) : CmEX from Clavibacter michiganensis = 72.4%: 27.6%, with no synergy of beta-glucosidase on degradation of alkaline pretreated rice straw. Under these conditions, the reducing sugar yield was 163.6% compared with the reaction containing cellulase alone. This work demonstrated the potential benefit of novel bacterial expansins on enhancing cellulose degradation efficiency in lignocellulosic biomass degradation. (C) 2014 Elsevier Ltd. All rights reserved.