This paper presents the study of the metals removal mechanisms in microcosm upflow constructed wetlands used for polishing of synthetic electroplating wastewater. Four types of columns were used: with and without plants and with different bed media (peat or gravel). The main design goal of the columns with peat was to promote precipitation of metals with biogenic sulfides produced by sulfate-reducing bacteria. The feed of the system contained mainly Cu, Ni, Pb, Zn, sulfates and cyanides. The substrate from the microcosm was sampled after cessation of the experiment (53 weeks) from the bottom and top layers of selected columns and was analyzed using sequential extraction procedure and scanning electron microscopy-energy-dispersive X-ray spectroscopy method. Additionally, solids retained on filter surface after filtering water samples withdrawn from the water layer covering the bed media in selected columns were analyzed by the latter method. The obtained results showed that the intended process for metals removal, which was precipitation of metal sulfides, was responsible for binding only a minor fraction of metals. The major portion of metals was present as exchangeable and reducible fraction. The presented study suggests that the onset of the conditions favorable for precipitation of sulfides may not occur during short-term experiment. It may stem from the specific characteristics of the experiment. It is suggested that short-term studies should not be extrapolated to predict long-term behavior of the system. (C) 2013 Elsevier B.V. All rights reserved.