Docosahexaenoic acid (DNA), one of the poly unsaturated fatty acids in omega-3 series, has attracted the attention of functional food industries due to its significant benefits to human health. DNA is often microencapsulated with modified starch and whey protein concentrate (WPC) to reduce oxidation, to improve stability during storage, and to mask any unpleasant odour. Spray drying is a common method to manufacture encapsulated powders. To the best of our knowledge, there is yet any work reported On the drying behaviour of the DNA emulsions. In fact, there is no reported literature on droplet drying behaviour, especially drying kinetics, for fish/algae oil in water emulsion system. Here, the drying behaviour of different kinds of DNA-containing emulsion droplets with varying concentrations and wall-materials were investigated via a single droplet drying device utilising the glass-filament approach to yield representative kinetics data. The changes of droplet size, moisture content, and droplet/particle temperature during drying were recorded, with the Reaction Engineering Approach (REA) employed to correlate the data. The related mechanisms were also discussed. The model has been shown to provide an accurate description of drying behaviour of different systems. This model can be implemented in dryer-wide simulation software to investigate large scale operations, thus the current work has provided the essential basis for future large scale explorations. (C) 2013 Elsevier Ltd. All rights reserved.