Highly sensitive SERS substrates capable of detecting less than a hundred molecules were prepared via TIJ technology. Films were prepared by printing silver nanoparticles (Ag NP) suspensions on quartz and other surfaces. Prepared substrates were characterized by UV-Vis spectrophotometry. Morphological evolution of films was monitored by atomic force microscopy. Inhomogeneous coverage of Ag NP was obtained for a single deposition, while a more uniform distribution of Ag NP was obtained when the number of depositions increased. SERS performance of the prepared SERS substrates was evaluated using p-aminobenzenethiol as a probe molecule. An estimated enhancement factor of 9.0 x 10(12) was obtained. (C) 2012 Elsevier B.V. All rights reserved.