A novel copolymer based on 2-aminothiazole (AT) and 2-amino-4-thiazoleacetic acid (ATA) is electrochemically synthesized and then characterized using UV-visible absorption spectroscopy (UV-vis), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and electrochemical techniques. The results confirmed that the obtained polymer was a copolymer rather than a blend or a composite of the respective homopolymers and could improve the electrochemical response of both positively and negatively charged analytes. Therefore, the copolythiazole film was applied to determine ascorbic acid (AA), dopamine (DA) and uric acid (UA). It is found that the peak separating degrees and peak currents of AA, DA and UA at copolythiazole modified electrode were much better than those at bare GCE. Under the optimum conditions, the linear calibration curves were obtained in the range of 10-2000 mu M for AA, 1-150 mu M for DA, 1-180 mu M for UA. The detection limits of AA, DA and UA were 2, 0.04 and 0.4 mu M, respectively (S/N = 3). The practical application of the modified electrode was demonstrated by the determination of UA in urine sample. (C) 2011 Elsevier Ltd. All rights reserved.