A novel hydrogen peroxide (H2O2) sensor has been fabricated by electrodepositing silver on glassy carbon electrode modified with layered double hydroxides (LDHs). Electrochemical characterizations demonstrate that the introduction of LDH substrate can enhance the electrocatalytic performance toward H2O2 of the sensor. Morphology characterization indicates that dendritic Ag nanostructure is present on the obtained electrodes by using LDHs as substrate. The growth mechanism of Ag nanodendrites is discussed using diffusion-limited aggregation model. After optimization of experimental conditions, a H2O2 sensor with a wide linear range (10 mu M to 19.33 mM) and a low detection limit (2.2 mu M) has been obtained. In addition, this sensor also exhibits good selectivity and reproducibility. This novel hierarchical structured sensor broadens the application of LDHs in electrochemical fields and provides a simple and feasible approach to meet the advanced application demand for H2O2 detection. (C) 2012 Elsevier Ltd. All rights reserved.