A novel reusable molecularly imprinted polymer (MIP) assembled on a polymeric layer of carboxylated poly(vinyl chloride) (PVC-COOH) for myoglobin (Myo) detection was developed. This polymer was casted on the gold working area of a screen printed electrode (Au-SPE), creating a novel disposable device relying on plastic antibodies. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and Fourier transform infrared spectroscopy (FTIR) studies confirmed the surface modification. The MIP/Au-SPE devices displayed a linear behaviour in EIS from 0.852 to 4.26 mu g mL(-1), of positive slope 6.50 +/- 1.48 (k Omega mL mu g(-1)). The limit of detection was 2.25 mu g mL(-1). Square wave voltammetric (SWV) assays were made in parallel and showed linear responses between 1.1 and 2.98 mu g mL(-1). A current decrease was observed against Myo concentration, producing average slopes of -0.28 +/- 0.038 mu A mL mu g(-1). MIP/Au-SPE also showed good results in terms of selectivity. The error% found for each interfering species were 7% for troponin T (TnT), 11% for bovine serum albumin (BSA) and 2% for creatine kinase MB (CKMB), respectively. Overall, the technical modification over the Au-SPE was found a suitable approach for screening Myo in biological fluids. (C) 2013 Elsevier Ltd. All rights reserved.