In this paper, electrochemical method was used to investigate the interactions between DNA and amphiphilic ferrocenylimine. The method was developed using the system of electrode modified with the amphiphilic ferrocenylimine LB films. Electrochemical results obtained from amphiphilic ferrocenylimine LB films modified electrode suggested that the forrocenium derivative could interact strongly with dsDNA. It was observed that the binding of amphiphilic ferrocenylimine to DNA led to a significant peak current decrease of amphiphilic ferrocenylimine LB films modified electrode. The interactions between amphiphilic ferrocenylimine and four basic groups were also investigated, the results revealed that the basic groups cytosine, thymine and adenine could interact with forrocenium complex, but guanine showed no interaction with forrocenium complex. The order of interaction intensity for four basic-groups with forrocenium complex (from strong to feeble) is cytosine > thymine > adenine > guanine. Therefore, the amphiphilic ferrocenylimine interacted with DNA through the basic groups cytosine, thymine and adenine. (C) 2013 Elsevier Ltd. All rights reserved.