In the present study, a new, simple and highly sensitive electrochemical method is developed for the determination of clonazepam (CZP) based on a silver nanoparticles/multi walled carbon nanotubes nanocomposite modified glassy carbon electrode (AgNPs/MWCNTs/GCE). Cyclic voltammetry and differential pulse voltammetry (DPV) methods were used for the electrochemical studies and measurements. The obtained results show that the AgNPs/MWCNTs/GCE exhibits high electrocatalytic activity toward the reduction of CZP. During the electrochemical reduction of CZP, an irreversible cathodic peak appears at about -0.61 V vs. silver/silver chloride electrode. The DPV measurements confirmed that the reduction peak currents increased linearly with CZP concentration in the range of 5.0 x 10(-8) - 2.5 x 10(-6) M with a detection limit of 6.0 x 10(-9) M. The stability, reproducibility and repeatability of the nanocomposite modified electrode were checked and the obtained relative standard deviations showed that the AgNPs/MWCNTs/GCE had excellent stability, reproducibility and repeatability. Finally, the nanocomposite modified electrode was effectively applied for the determination of CZP in some real samples without any interference. Published by Elsevier Ltd.