Information hidden in the characteristics and relationship data of a cascade hydropower stations can be extracted by data-mining approaches to be operation rules and optimization support information. In this paper, with Three-gorge and Gezhouba cascade hydropower stations as an example, two operation rules are proposed due to different operation efficiency of water turbines and tight water volume and hydraulic relationship between two hydropower stations. The rules are applied to improve optimization model with more exact decision and state variables and constraints. They are also used in the population initiation step to develop better individuals with culture algorithm with differential evolution as an optimization method. In the case study, total feasible population and the best solution based on an initial population with an operation rule can be obtained with a shorter computation time than that of a pure random initiated population. Amount of electricity generation in a dispatch period with an operation rule also increases with an average increase rate of 0.025%. For a fixed water discharge process of Three-gorge hydropower station, there is a better rule to decide an operation plan of Gezhouba hydropower station in which total hydraulic head for electricity generation is optimized and distributed with inner-plant economic operation considered. (C) 2012 Elsevier Ltd. All rights reserved.