A recursion relation is formulated for the Green’s function for calculating the effective electron coupling in bridge-assisted electronic transfer systems, within the framework of the tight-binding Hamiltonian. The recursion expression relates the Green’s function of a chain bridge to that of the bridge that is one unit less. It is applicable regardless of the number of orbitals per unit. This method is applied to the system of a ferrocenylcarboxy-terminated alkanethiol on the Au(111) surface. At larger numbers of bridge units, the effective coupling strength shows an exponential decay as the number of methylene(-CH2-) units increases. This sequential formalism shows numerical stability even for a very lung chain bridge and, since it uses only small matrices, requires much less computer time for the calculation. Identical bridge units are not a requirement, and so the method can be applied to more complicated systems.