The visible light-operated saccharide-O-2 biofuel cell consisting of zinc chlorin-e(6) (ZnChl-e(6)) adsorbed on nanocrystalline TiO2 layer coated onto optical transparent conductive glass electrode (OTE) as an anode, platinum-coated OTE as a cathode, and the fuel solution containing sucrose as a saccharide, invertase, glucose dehydrogenase (GDH) and NAD(+) is studied as a new type biofuel cell. The short-circuit photocurrent (I-SC) and the open-circuit photovoltage (V-OC) of this cell are 9.0 mu A cm(-2) and 415 mV, respectively. The peaks in the photocurrent action spectrum of this cell are observed at 400 and 800 nm and the incident photon-to-current efficiency (IPCE) values at 400 and 800 nm are estimated to be ca. 17.3% and 10.6%. Thus, a new type of visible light-operated saccharide-O-2 biofuel cell with the visible and near IR photosensitization of ZnChl-e(6) molecules on nanocrystalline TiO2 film electrode is accomplished. (c) 2008 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.