Platinum is employed as anode catalyst for low temperature electro-oxidation of glucose in direct glucose fuel cell (DGFC), but it suffers from poisoning by intermediate oxidation products. In the present investigation, palladium and gold precursors are added with platinum precursor to form low metal loading (similar to 15-20% by wt.) carbon supported catalyst by NaBH4 reduction technique. The prepared PtPdAu/C (metal ratio 1:1:1) and PdPt/C (metal ratio 4:1) catalysts are tested in DGFC. The Physical characterization of electro-catalysts by scanning electron microscope, transmission electron microscope, energy dispersive X-ray, X-ray diffraction and thermo-gravimetric analysis confirms the formation of nano-sized metal particles on carbon substrate with two prominent homogeneous bi- or tri-metallic crystal phases for PtPdAu/C. The cyclic voltammetry studies carried out for glucose (0.05 M) oxidation in (0.5 M KOH) alkaline medium shows the metal catalysts can efficiently electro-oxidize glucose. The catalysts tested as anode in a batch type DGFC using commercial activated charcoal as cathode produced peak power density of 0.52 mW cm(-2) for both PdPt/C and PtPclAu/C in 0.3 M glucose in 1 M KOH solution. Copyright (C) 2011, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.