Hydrogen and electric vehicle technologies are being considered as possible solutions to mitigate environmental burdens and fossil fuel dependency. Life cycle analysis (LCA) of energy use and emissions has been used with alternative vehicle technologies to assess the Well-to-Wheel (WTW) fuel cycle or the Cradle-to-Grave (CTG) cycle of a vehicle's materials. Fuel infrastructures, however, have thus far been neglected. This study presents an approach to evaluate energy use and CO2 emissions associated with the construction, maintenance and decommissioning of energy supply infrastructures using the Portuguese transportation system as a case study. Five light-duty vehicle technologies are considered: conventional gasoline and diesel (ICE), pure electric (EV), fuel cell hybrid (FCHEV) and fuel cell plug-in hybrid (FC-PHEV). With regard to hydrogen supply, two pathways are analysed: centralised steam methane reforming (SMR) and on-site electrolysis conversion. Fast, normal and home options are considered for electric chargers. We conclude that energy supply infrastructures for FC vehicles are the most intensive with 0.03-0.53 MJ(eq)/MJ emitting 0.7-27.3 g CO2eq/MJ of final fuel. While fossil fuel infrastructures may be considered negligible (presenting values below 2.5%), alternative technologies are not negligible when their overall LCA contribution is considered. EV and FCHEV using electrolysis report the highest infrastructure impact from emissions with approximately 8.4% and 8.3%, respectively. Overall contributions including uncertainty do not go beyond 12%. Copyright (C) 2012, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.