In this investigation, water in a single-cell proton exchange membrane (PEM) fuel cell was managed using saturated hydrogen and dry air. The experiment was conducted at temperatures of 40, 50 and 60 degrees C and pressures of 1 and 1.5 bar at both the anode and cathode gas inlets. The feed velocities of hydrogen and air were fixed at 3 and 6 L min(-1), respectively. After reaching steady-state conditions, the relative humidity along the single serpentine gas channel was measured. From the experimental data, water transport properties were characterized based on a membrane hydration model. The electro-osmotic drag coefficient, water diffusion coefficient, membrane ionic conductivity and water back-diffusion flux were significantly influenced by the water content in the membrane of the PEM fuel cell. The water content depended on the relative humidity profile along the gas channel. In this investigation, a negative value for the water back-diffusion flux was measured; thus, the transport of water from the cathode to the anode did not occur. This phenomenon was due to the large water concentration gradient between the anode and cathode. Therefore, this strategy successfully prevented flooding in the PEM fuel cell. Copyright (C) 2012, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.