A thin amine-functionalized MIL-53 membrane with high permeability of hydrogen was successfully prepared on a porous alpha-Al2O3 support by using the secondary growth method. Seeded alpha-Al2O3 supports were prepared by a dip-coating technique. In contrast, a discontinuous membrane was obtained by using unseeded support under the same synthesis conditions, implying that the seeds play the key role in the formation of compact membranes. The resulting compact membranes were measured by X-ray diffraction (XRD), scanning electron microscopy (SEM) and single gas permeation testing. Results showed that the thickness of the as-prepared membrane was around 2 similar to 4 mu m. Hydrogen permeance of the as-prepared membrane reached a remarkable value of 1.5 x 10(-5) mol m(-2) s(-1).Pa-1 at room temperature under a 0.1 MPa pressure drop. The ideal H-2/CO2 selectivity was found to be 4.4. In addition, the influence of seeding solution on the membrane performance was investigated. We found that the membrane permeance decreased and the ideal selectivity increased when the seeding solution content was increased. Copyright (C) 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.