In this work low-loaded platinum bi-metallic amorphous alloys based on Ni59Nb40Pt0.6X0.4 (X = Ru, Sn) were studied as electro-catalytic anodes in PEMFCs. The electro-catalysts were produced by Mechanical Alloying (MA) using a planetary ball-mill during 40 h and used as anodes onto Nafion (R) (DuPont) perfluorinated membranes (Nafion (R) 117, Nafion (R) 115 and Nafion (R) XL). In addition, two active areas of fuel cells were used namely 9 and 25 cm(2). These electro-catalysts were characterized by both physical and chemical techniques, such as Differential Scanning Calorimetry (DSC), X-ray Diffraction (XRD) and Energy Dispersive X-ray Spectroscopy (EDX). Concerning the performance towards the Hydrogen Oxidation Reaction (HOR), the results showed that 25 cm(2) of active area of fuel cell with Ni59Nb40P-t(0.6)Ru(0.4) alloy and the Membrane Electrode Assembly (MEA) based on the Nafion (R) 115 membrane exhibited the best catalytic activity (2083.59 A g(-1) Pt), while for the Ni59Nb40Pt0.6Sn0.4 catalyst the Nafion (R) XL membrane was more suitable (1570.82 A g-1 Pt). Copyright (c) 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.