In this study the tolerance of the alkalithermophile Caloramator celer towards substrate (glucose) and soluble end product (acetate, formate and ethanol) inhibition was assessed employing nonlinear inhibition models. In addition, the effects of subinhibitory concentrations of end products on fermentative metabolism and regulation of 12 key genes involved in pyruvate catabolism were studied. Optimal growth and H-2 production were found at 50 mM of glucose and the critical substrate concentration was observed at 290-360 mM. Two inhibition models revealed that ethanol had a higher inhibitory effect on growth rate, whereas H-2 production kinetics was more sensitive towards increasing concentrations of acetate and formate. Acetate, the main soluble metabolite of the fermentation, inhibited the H-2 production by increasing the ionic strength in the medium. Subinhibitory concentrations of soluble end products induced changes in the metabolite profile of C. celer, specifically exogenous acetate (80 mM) and ethanol (40 mM) slightly increased the H-2 yield by 4 and 7%, respectively. However, despite the observed metabolic shifts, gene regulation was minimal and not always in agreement with the measured product yields. Overall, the results suggest that further optimization of the H-2 production process from C. celer should focus on methods to evolve adapted osmotolerant strains and/or remove soluble metabolites, especially acetate, from the culture. Copyright (C) 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.