α, β, γ-chitin were isolated from natural resources by treatment with NaOH solution and for deprotenization, and then by treatment with dilute HCl solution for demineralization. The chitin gel matrices were prepared using α, β, γ-chitin for application of drug delivery systems. In this study, α, β, γ-chitin were isolated from crab shell, squid pen and beetles of cuticle, respectively, and physicochemical properties were investigated using fourier transform infrared spectrometer, X-ray diffractometer, and differential scanning calorimeter. And then the chitin gel matrices were prepared using α, β, γ-chitin and dissolution characteristics of drug from chitin gel matrices were investigated. The dissolution profiles of α, β, γ-chitin gel matrices were determined by means of available compendium. Dissolution efficiency of these gel matrices were observed at 37±0.5 ℃.