Experimental investigation of two-phase flow patterns for refrigerant R-134a and air-water in horizontal tubes with inside diameter from 1.0 to 3.0 mm was performed. The air-water test results agree very well with previous work. However, R-134a flow leads to a shift in the slug to annular transition to lower value of gas velocity. The locations of bubble to plug and slug flow transition are also significantly affected by the working fluids properties. We concluded that, in addition to buoyant force and turbulent fluctuations, surface tension force is also an important parameter for flow pattern determination in small tubes. Surface tension force causes the system to act to minimize its interfacial area. It tends to keep bubbles retaining its circular shapes and also to keep the liquid holdup between the tube walls to retard the transition from slug to annular. Since the surface tension of air-water is much larger than that of R-134a, it makes the intermittent to bubble flow transition occurs earlier for air-water than for R-134a. And also leads to a shift in the slug to annular transition to lower value of gas velocity for R-134a.