Herein, the potential of Fourier transform infrared spectroscopy (FTIR) spectroscopy coupled with principal component analysis and artificial neural networks to discriminate butters obtained from milk creams collected in different seasons (spring, summer or winter) and produced from two feeding regimens (traditional or unifeed) in the Parmigiano Reggiano cheese area was investigated. The predictive ability of neural networks to predict the season of production was 100%. The favorable results obtained by artificial neural network (ANN) analysis were in agreement with those reported by principal component analysis (PCA) analysis. The ability of ANNs to predict the feeding regimens was 90.0%, 75.0% and 75.0%, respectively, for samples collected in spring, summer and winter. These results also confirm that the method is highly suitable for its intended purpose. (C) 2011 Elsevier Ltd. All rights reserved.