The physical properties of a composite film prepared from barley bran protein and gelatin (BBG) were investigated. Tensile strength (TS) and elongation at break (E) values of the BBG film decreased as barley bran protein content increased. TS increased with increasing gelatin content, but E values decreased. The optimal conditions for the preparation of the BBG film were 3 g barley bran protein, 3 g gelatin, and 1 g sorbitol in 100 mL film-forming solution. In order to inhibit the growth of pathogenic bacteria, a BBG film containing grapefruit seed extract (GSE) was prepared. After 15 days of storage, populations of Escherichia coli O157:H7 and Listerio monocyrogenes inoculated on salmon packaged with the BBG film containing GSE decreased by 0.53 and 0.50 log CFU/g, respectively, compared to the control. Also, packing salmon with the BBG film containing GSE decreased the peroxide value and thiobarbituric acid value by 23.0% and 23.4%, respectively. (C) 2012 Elsevier Ltd. All rights reserved.