Microfiltration (MF) is classified as a non-thermal process for the fruit juice industry. It could provide a better preservation of the phytochemical property and flavor of the juice. This work aimed to study the stability of phytochemical properties including vitamin C, total phenolic content, antioxidant capacity (2-Diphenly-1-picrylhydrazyl: DPPH, free radical scavenging capacity and Oxygen Radical Absorbance Capacity: ORAC assays), microbial and chemical-physical (color, browning index, pH and total soluble solid) properties of MF-clarified pineapple juice during storage at various temperatures (i.e. 4, 27, and 37 degrees C). The juices were clarified by microfiltration using hollow fiber module. The results showed that most of the phytochemical properties and soluble components were retained in the juice after microfiltration. No microbial growth was detected after 6 months of storage. The storage time and temperature did not affect total soluble solids and pH (P>0.05). The color (L*) of clarified juice stored at 4 degrees C was lighter than the juices stored at higher temperature levels (P<0.05). The phytochemical properties and total phenol content of the juice significantly decreased as storage time and temperature increased (P<0.05). Vitamin C content was the attribute that affected storage time and temperature most as indicated by reaction rate constant and activated energy. Storage of non-thermally pasteurized and clarified pineapple juice at 4 degrees C was the most suitable since it allowed the best quality preservation. (C) 2012 Elsevier Ltd. All rights reserved.