A hybrid process combining solubilization via sulfuric acid produced by sulfur-oxidizing bacteria with precipitation via sulfide produced by sulfate-reducing bacteria was investigated to isolate soilborne metal contaminants as purified metal-sulfides. The highly efficient two-step acidification process involved bioproduction of sulfuric acid in a culture medium containing 30% (v/v) of sludge filtrate (SF). Soil was added to the culture after maximum acid production. Solubilization efficiencies of 95% for Zn, 76% for Cu and 97% for Cd were achieved after 16 days. At pH 1.9, 3.0 and 4.0, 99% of Cu2+, 96% of Cd3+ and 93% of Zn2+, respectively, were precipitated from the soil leachate by sulfide transported from sulfidogenic bioreactor via N-2 sparging, resulting in final effluent metal contents at the ppb-level. The introduction of SF did not affect the precipitation kinetics and purity of the recovered precipitates. Ultimately, 75% of Cu and 86% of Zn were recovered from the soil as pure CuS and ZnS (confirmed by SEM-EDS and XRD). These results demonstrate the potential of the integrated method for the selective production of valuable metals from metal contamination in soils. (C) 2012 Elsevier B.V. All rights reserved.