Adsorption Properties of Bovine Serum Albumin on Porous Polymer Microgels

Kim KS; Kang SH

Korea Polymer Journal, Vol.6, No.3, 235-240, August, 1998

The adsorption of bovine serum albumin (BSA) on monodisperse porous poly(butyl methacrylate-divinylbenzene) (PBMA), poly(4-vinylpyridine-divinylbenzene) (PVP), and poly (acrylonitrile-ethylene glycol dimethacrylate) (PAN) microgels with different surface properties was studied. The adsorption isotherm of BSA on these micogels was measured as functions of pH and ionic strength. The effect of pH dependence for BSA adsorption on the microgels, which differ from the surface properties, showed the maximum adsorption at the isoelectric point (pI=pH 4.8-5.3) of BSA. The PBMA microgels showed the highest adsorption isotherm for BSA over the whole range of pH and ionic strength. Also, the desorption isotherm on PBMA microgels over the whole range of pH showed hydrophobic polymer microgels are more dependent on the hydrophobic interaction than electrostatic effect. The BSA adsorption on PBMA microgels was afected by the pH and ionic strength less than on PVP and PAN microgels. The experimental asorption isotherm of the micrgels for BSA better fitted to the Langmuir equation than Freundlich equation.

[References]

Pittner F, Miron T, Pittner G, Wilchek M, J. Solid Phase Biochem., 5, 147 (1980)
Pittner F, Miron T, Pittner G, Wilchek M, J. Solid Phase Biochem., 5, 167 (1980)
Pecht I, Mazurek N, Petrank A, Margel S, Targeting of Drugs, Ed. by Gregoriadis, Senior and Trouet, Plenum, New York, pp. 109-123 (1982)
Tokes ZA, Rogers KE, Rembaum A, Proc. Nat. Acad. Sci. U.S.A., 79, 2026 (1982)
Margel S, Offarim M, Anal. Biochem., 128, 342 (1982)
Gray GR, Anal. Chem., 52, 9R (1980)
Cuatrecasas P, J. Biol. Chem., 245, 3059 (1970)
Hradil J, Wojaczynska M, Svec F, Kolarz BN, React. Polym., 4, 277 (1986)
Nakamura K, Hirai Y, Kitano H, Ise N, Biotechnol. Bioeng., 30, 216 (1987)
Kulin LI, Flodin P, J. Chromatogr., 514, 1 (1990)
Ellingsen T, Aune O, Ugelstad J, Hagen S, J. Chromatogr., 535, 147 (1990)
Li WH, Stover HD, Hamielec AE, J. Polym. Sci. A: Polym. Chem., 32(11), 2029 (1994)
Wang QC, Svec F, Frechet JM, J. Polym. Sci. A: Polym. Chem., 32(13), 2577 (1994)
Seidl J, Malinsky J, Dusek K, Heitz W, Adv. Polym. Sci., 5, 113 (1967)
Kondo A, Oku S, Higashitani K, Biotechnol. Bioeng., 37, 537 (1991)
Kitano H, Nakamura K, Biotechnol. Bioeng., 31, 547 (1988)
Kitano H, Nakamura K, Biotechnol. Bioeng., 37, 608 (1991)
Yoshida H, Nishihara H, Biotechnol. Bioeng., 41, 280 (1993)
Yoshida H, Nishihara H, Kataoka T, Biotechnol. Bioeng., 43(11), 1087 (1994)
Kim KS, Kang SH, Shin JS, J. Ind. Eng. Chem., 2(1), 45 (1996)
Brunaur S, Emmett P, Teller E, J. Am. Chem. Soc., 60, 309 (1938)

[1] Pittner F, Miron T, Pittner G, Wilchek M, J. Solid Phase Biochem., 5, 147 (1980)

[2] Pittner F, Miron T, Pittner G, Wilchek M, J. Solid Phase Biochem., 5, 167 (1980)

[3] Pecht I, Mazurek N, Petrank A, Margel S, Targeting of Drugs, Ed. by Gregoriadis, Senior and Trouet, Plenum, New York, pp. 109-123 (1982)

[4] Tokes ZA, Rogers KE, Rembaum A, Proc. Nat. Acad. Sci. U.S.A., 79, 2026 (1982)

[5] Margel S, Offarim M, Anal. Biochem., 128, 342 (1982)

[6] Gray GR, Anal. Chem., 52, 9R (1980)

[7] Cuatrecasas P, J. Biol. Chem., 245, 3059 (1970)

[8] Hradil J, Wojaczynska M, Svec F, Kolarz BN, React. Polym., 4, 277 (1986)

[9] Nakamura K, Hirai Y, Kitano H, Ise N, Biotechnol. Bioeng., 30, 216 (1987)

[10] Kulin LI, Flodin P, J. Chromatogr., 514, 1 (1990)

[11] Ellingsen T, Aune O, Ugelstad J, Hagen S, J. Chromatogr., 535, 147 (1990)

[12] Li WH, Stover HD, Hamielec AE, J. Polym. Sci. A: Polym. Chem., 32(11), 2029 (1994)

[13] Wang QC, Svec F, Frechet JM, J. Polym. Sci. A: Polym. Chem., 32(13), 2577 (1994)

[14] Seidl J, Malinsky J, Dusek K, Heitz W, Adv. Polym. Sci., 5, 113 (1967)

[15] Kondo A, Oku S, Higashitani K, Biotechnol. Bioeng., 37, 537 (1991)

[16] Kitano H, Nakamura K, Biotechnol. Bioeng., 31, 547 (1988)

[17] Kitano H, Nakamura K, Biotechnol. Bioeng., 37, 608 (1991)

[18] Yoshida H, Nishihara H, Biotechnol. Bioeng., 41, 280 (1993)

[19] Yoshida H, Nishihara H, Kataoka T, Biotechnol. Bioeng., 43(11), 1087 (1994)

[20] Kim KS, Kang SH, Shin JS, J. Ind. Eng. Chem., 2(1), 45 (1996)

[21] Brunaur S, Emmett P, Teller E, J. Am. Chem. Soc., 60, 309 (1938)