Alpha spectrometry and solid state nuclear track detectors (SSNTDs) are used for monitoring ultra-trace amount of alpha emitting actinides in different aqueous streams. However, these techniques have limitations i.e. alpha spectrometry requires a preconcentration step and SSNTDs are not chemically selective. Therefore, a thin polymer inclusion membrane (PIM) supported on silanized glass was developed for pre-concentraion and determination of ultra-trace concentration of actinides by alpha-spectrometry and SSNTDs. PIMs were formed by spin coating on hydrophobic glass slide or solvent casting to form thin and self-supported membranes, respectively. Sorption experiments indicated that uptakes of actinides in the PIM were highly dependent on acidity of solution i.e. Am(III) sorbed up to 0.1 mol L-1 HNO3, U(VI) up to 0.5 mol L-1 HNO3 and Pu(IV) from HNO3 concentration as high as 4 mol L-1. A scheme was developed for selective sorption of target actinide in the PIM by adjusting acidity and oxidation state of actinide. The actinides sorbed in PIMs were quantified by alpha spectrometry and SSNTDs. For SSNTDs, neutron induced fission-fragment tracks and alpha-particle tracks were registered in Garware polyester and CR-39 for quantifications of natural uranium and alpha-emitting actinides (Am-241/(239)pu/U-233), respectively. Finally, the membranes were tested to quantify Pu in 4 mol L-1 HNO3 solutions and synthetic urine samples. (C) 2013 Elsevier B.V. All rights reserved.