A simple, proficient, one-pot green chemical method for the biosynthesis of gold nanoparticles (AuNPs) with protein egg albumin by microwaves, which produce novel gold nanobiocatalyst (AuNBC) and it was characterized by FT-IR, UV-vis, TEM and DLS, which exhibited excellent stability over a wide range of pH and temperature. The catalytic activity of the as-synthesized AuNBC was quantified by synthesizing sodium benzoate from the one-pot oxidation of benzyl alcohol using gold nanobiocatalyst under microwave heating, which was stronger than AuNPs prepared by conventional chemical methods. The protocol is environmentally benign with non-toxic reagents, high recyclability and high efficiency. (C) 2013 Elsevier B.V. All rights reserved.