Fabrication of Biomimetic PCL Scaffold Using Rapid Prototyping for Bone Tissue Engineering

Su A. Park; Jung Bok Lee; Yang Eun Kim; Ji Eun Kim; Jun Hee Lee; Jung-Woog Shin; Il Keun Kwon; WanDoo Kim

Macromolecular Research, Vol.22, No.8, 882-887, August, 2014

DOI10.1007/s13233-014-2119-5 Export Citation

Fabrication of Biomimetic PCL Scaffold Using Rapid Prototyping for Bone Tissue Engineering

Su A. Park^{1, †}, Jung Bok Lee^{2, 3}, Yang Eun Kim¹, Ji Eun Kim^{2, 4}, Jun Hee Lee¹, Jung-Woog Shin⁵, Il Keun Kwon^{2, 4}, and WanDoo Kim¹

¹Nature-Inspired Mechanical System Team, Nano Convergence & Manufacturing Systems Research Division, Korea Institute of Machinery & Materials (KIMM), Daejeon, 305-343, Korea
²Department of Maxillofacial Biomedical Engineering, School of Dentistry, Kyung Hee University, Seoul, 130-701, Korea
³Department of Biomedical Engineering, Vanderbilt University, Nashville, Tennessee, USA
⁴, Korea
⁵Department of Biomedical Engineering, Inje University, Gyeongnam, 621-749, Korea

E-mail:

We report the fabrication of a porous three-dimensional (3D) scaffold using a 3D plotting system, with applications in bone tissue engineering. Biomimetic surface coatings of hydroxyapatite were formed using concentrated simulated body fluid (SBF). The mineralized scaffold had a uniform interconnected porous structure. The apatite that was formed on the surface of the scaffold was characterized by using energy dispersive spectroscopy (EDS), Xray powder diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The biological properties of the scaffold were evaluated using WST-1 assays of alkaline phosphatase (ALP) activity and gene expression of D1 mouse mesenchymal stem cells. The results show that the biomimetic scaffolds exhibited good apatite-forming properties, with favorable cell proliferation and differentiation for bone formation. Therefore, these biomimetic scaffolds with hydroxyapatite coatings have potential applications in bone regeneration.

Keywords:3D plotting system;hydroxyapatite;biomimetic;simulated body fluid.

[References]

Rezwan K, Chen QZ, Blaker JJ, Boccaccini AR, Biomaterials, 27, 3413 (2006)
Robling AG, Castillo AB, Turner CH, Annu. Rev. Biomed. Eng., 8, 455 (2006)
Salerno A, Oliviero M, Maio ED, Iannace S, Netti PA, J. Mater. Sci. Mater. Med., 20, 2043 (2009)
Hutmacher DW, Schantz T, Zein I, Ng KW, Teoh SH, Tan KC, J. Biomed. Mater. Res., 55, 203 (2001)
Woodfield TBF, Malda J, de Wijn J, Peters F, Riesle J, van Blitterswijk CA, Biomaterials, 25, 4149 (2004)
Albrecht DR, Underhill GH, Wassermann TB, Sah RL, Bhatia SN, Nat. Methods, 3, 369 (2006)
Leong KF, Chua CK, Sudarmadji N, Yeong WY, J. Mech. Behav. Biomed. Mater., 1, 140 (2008)
Zein I, Hutmacher DW, Tan KC, Teoh SH, Biomaterials, 23, 1169 (2002)
Giordano RA, Wu BM, Borland SW, Cima LG, Sachs EM, Cima MJ, J. Biomater. Sci. Polym. Ed., 8, 63 (1997)
Landers R, Hubner U, Schmelzeisen R, Mulhaupt R, Biomaterials, 23, 4437 (2002)
Park S, Kim G, Jeon YC, Koh Y, Kim W, J. Mater. Sci. Mater. Med., 20, 229 (2009)
Kim JY, Yoon JJ, Park EK, Kim DS, Kim SY, Cho DW, Biofabrication, 1, 015002 (2009)
Shin M, Yoshimoto H, Vacanti JP, Tissue Eng., 10, 33 (2004)
Causa F, Netti PA, Ambrosio L, Ciapetti G, Baldini N, Pagani S, Martini D, Giunti A, J. Biomed. Mater. Res. A, 76, 151 (2006)
Shim JH, Moon TS, Yun KJ, Jeon YC, Jeong CM, Cho DW, Huh JB, J. Mater. Sci. Mater. Med., 23, 2993 (2012)
Shim JH, Huh JB, Park JY, Jeon YC, Kang SS, Kim JY, Rhie JW, Cho DW, Tissue Eng. Part A, 19, 317 (2013)
Kretlow JD, Mikos AG, Tissue Eng., 13, 927 (2007)
Kokubo T, Ito S, Huang ZT, Hayashi T, Sakka S, Kitsugi T, Yamamuro T, J. Biomed. Mater. Res., 24, 331 (1990)
Cho SB, Nakanishi K, Kokubo T, Soga N, Ohtsuki C, Nakamura T, J. Biomed. Mater. Res., 33, 145 (1996)
Kokubo T, Takadama H, Biomaterials, 27, 2907 (2006)
Lee TJ, Bhang SH, La WG, Kwon SH, Shin JY, Yoon HH, Shin HS, Cho DW, Kim BS, Ann. Plast. Surg., 70, 98 (2013)
Fu S, Yang L, Fan J, Wen Q, Lin S, Wang B, Chen L, Meng X, Chen Y, Wu J, Colloids Surf. B: Biointerfaces, 107, 167 (2013)
Li WJ, Laurencin CT, Caterson EJ, Tuan RS, Ko FK, J. Biomed. Mater. Res., 60, 613 (2002)
Zeltinger J, Landeen LK, Alexader HG, Kidd ID, Sibanda B, Tissue Eng., 7, 557 (2001)
Chen QZ, Efthymiou A, Salih V, Boccaccini AR, J. Biomed. Mater. Res. A, 84, 1049 (2008)
Young MF, Kerr JM, Ibaraki K, Heegaard AM, Robey PG, Clin. Orthop. Relat. Res., 281, 275 (1992)
Vines JB, Lim DJ, Anderson JM, Jun HW, Acta Biomater., 8, 4053 (2012)
Sowa H, Kaji H, Canaffi L, Hendy GN, Tsukamoto T, Yamaguchi T, Miyazono K, Sugimoto T, Chihara K, J. Biol. Chem., 278, 21058 (2003)

[Referenced By]

[1] Rezwan K, Chen QZ, Blaker JJ, Boccaccini AR, Biomaterials, 27, 3413 (2006)

[2] Robling AG, Castillo AB, Turner CH, Annu. Rev. Biomed. Eng., 8, 455 (2006)

[3] Salerno A, Oliviero M, Maio ED, Iannace S, Netti PA, J. Mater. Sci. Mater. Med., 20, 2043 (2009)

[4] Hutmacher DW, Schantz T, Zein I, Ng KW, Teoh SH, Tan KC, J. Biomed. Mater. Res., 55, 203 (2001)

[5] Woodfield TBF, Malda J, de Wijn J, Peters F, Riesle J, van Blitterswijk CA, Biomaterials, 25, 4149 (2004)

[6] Albrecht DR, Underhill GH, Wassermann TB, Sah RL, Bhatia SN, Nat. Methods, 3, 369 (2006)

[7] Leong KF, Chua CK, Sudarmadji N, Yeong WY, J. Mech. Behav. Biomed. Mater., 1, 140 (2008)

[8] Zein I, Hutmacher DW, Tan KC, Teoh SH, Biomaterials, 23, 1169 (2002)

[9] Giordano RA, Wu BM, Borland SW, Cima LG, Sachs EM, Cima MJ, J. Biomater. Sci. Polym. Ed., 8, 63 (1997)

[10] Landers R, Hubner U, Schmelzeisen R, Mulhaupt R, Biomaterials, 23, 4437 (2002)

[11] Park S, Kim G, Jeon YC, Koh Y, Kim W, J. Mater. Sci. Mater. Med., 20, 229 (2009)

[12] Kim JY, Yoon JJ, Park EK, Kim DS, Kim SY, Cho DW, Biofabrication, 1, 015002 (2009)

[13] Shin M, Yoshimoto H, Vacanti JP, Tissue Eng., 10, 33 (2004)

[14] Causa F, Netti PA, Ambrosio L, Ciapetti G, Baldini N, Pagani S, Martini D, Giunti A, J. Biomed. Mater. Res. A, 76, 151 (2006)

[15] Shim JH, Moon TS, Yun KJ, Jeon YC, Jeong CM, Cho DW, Huh JB, J. Mater. Sci. Mater. Med., 23, 2993 (2012)

[16] Shim JH, Huh JB, Park JY, Jeon YC, Kang SS, Kim JY, Rhie JW, Cho DW, Tissue Eng. Part A, 19, 317 (2013)

[17] Kretlow JD, Mikos AG, Tissue Eng., 13, 927 (2007)

[18] Kokubo T, Ito S, Huang ZT, Hayashi T, Sakka S, Kitsugi T, Yamamuro T, J. Biomed. Mater. Res., 24, 331 (1990)

[19] Cho SB, Nakanishi K, Kokubo T, Soga N, Ohtsuki C, Nakamura T, J. Biomed. Mater. Res., 33, 145 (1996)

[20] Kokubo T, Takadama H, Biomaterials, 27, 2907 (2006)

[21] Lee TJ, Bhang SH, La WG, Kwon SH, Shin JY, Yoon HH, Shin HS, Cho DW, Kim BS, Ann. Plast. Surg., 70, 98 (2013)

[22] Fu S, Yang L, Fan J, Wen Q, Lin S, Wang B, Chen L, Meng X, Chen Y, Wu J, Colloids Surf. B: Biointerfaces, 107, 167 (2013)

[23] Li WJ, Laurencin CT, Caterson EJ, Tuan RS, Ko FK, J. Biomed. Mater. Res., 60, 613 (2002)

[24] Zeltinger J, Landeen LK, Alexader HG, Kidd ID, Sibanda B, Tissue Eng., 7, 557 (2001)

[25] Chen QZ, Efthymiou A, Salih V, Boccaccini AR, J. Biomed. Mater. Res. A, 84, 1049 (2008)

[26] Young MF, Kerr JM, Ibaraki K, Heegaard AM, Robey PG, Clin. Orthop. Relat. Res., 281, 275 (1992)

[27] Vines JB, Lim DJ, Anderson JM, Jun HW, Acta Biomater., 8, 4053 (2012)

[28] Sowa H, Kaji H, Canaffi L, Hendy GN, Tsukamoto T, Yamaguchi T, Miyazono K, Sugimoto T, Chihara K, J. Biol. Chem., 278, 21058 (2003)