The enzyme salicylate hydroxylase was produced from Pseudomonas putida UUC-1 in an 80 dm(3) 16 h batch fermentation process yielding, similar to 9 K units per 60 dm(3). This enzyme preparation has been studied for its application in the construction of biosensor systems, e.g. carbon paste electrodes, screen-printed carbon electrodes, and disposable carbon enzyme electrodes, which will be used for the rapid estimation of salicylate in blood samples. The linear range of the disposable carbon enzyme electrode in response to salicylate was achieved to 1.8 mmol dm(-3).